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CNAγ is localized to synapses in hippocampal cultures. A, immunocytochemistry was performed on cultured rat hippocampal neurons with antibodies against rat CNAγ (affinity purified antisera) and vGlut1. Scale bar = 10 μm. B, mean pixel intensities of vGlut1 and CNAγ labeling were measured from 504 vGlut1-positive puncta from five neurons. Data were fit with linear regression (R2 = 0.39, p < 0.0001). Norm, normalized.
