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Myriocin treatment reduced mitochondrial ceramide after IR. WT mice were given MR via gavage (2 mg/kg) following 1 h of MCAO/3 h of reperfusion. WT mice control group received a vehicle control. Mitochondria were isolated from the ipsilateral (IR) and contralateral (Con) hemispheres after 1 h of MCAO/24 h of reperfusion, and ceramide species were determined by tandem MS. Data are means ± S.E., *, p < 0.05, n = 12.
