FIGURE 7.

Sirt3 gene ablation rescued IR-induced complex III activity defect and diminished ROS formation and protein carbonyls in cerebral mitochondria. Mitochondria were purified from the WT and Sirt3 KO mouse brain hemispheres ipsilateral (IR) and contralateral (Con) to damage following 1 h of MCAO/24 h of reperfusion. A, respiratory chain complex III enzyme activity was determined by measuring the reduction of cytochrome c (cyt c) as described under “Experimental Procedures.” Data are means ± S.E., *, p < 0.05, n = 6. B, ROS generation was measured in the presence of respiratory chain complex I substrate 5 mm glutamate plus 5 mm malate (glutamate + malate) and complex II substrate 10 mm succinate (succinate). Data are means ± S.E., *, p < 0.05, n = 8. Bottom panel, protein carbonyls were assessed as described under “Experimental Procedures.” Data are means ± S.E., *, p < 0.05, n = 8.