FIGURE 1.

HNSCC conditioned media polarizes myeloid cells toward an MDSC phenotype. (A) CD33⁺ cells were cultured in NM or HNSCC conditioned media (HN6, HN13) for 72 h and then analyzed by flow cytometry. (B) Dose-dependent induction of MDSC following culture in HNSCC conditioned media. CD33⁺ cells were cultured in serial dilutions of HN6 conditioned medium (CM) and (C) HN13 CM. Cells were first gated for CD33⁺ then for CD11b⁺, followed by a third gate for CD11b⁺HLA-DR^−/low. CD33 cells were isolated using MACS from PBMC separated by centrifugation gradient of peripheral blood. All samples were run in duplicates. CM, conditioned medium.