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. 2016 Jan 1;36(1):37–47. doi: 10.1089/jir.2015.0006

FIG. 1.

FIG. 1.

Specific depletion of euchromatic histone-lysine N-methyltransferase 2 (EHMT2) in fetal bovine fibroblast (FBF) cells potently induce type I interferon (IFN) transcripts. FBF cells were mock transfected or transfected with siRNAs targeting bovine EHMT2 or SetDB1. Cells were stimulated with 0.5 μg/mL of poly (I:C) and harvested 6 h later for quantitative real-time polymerase chain reaction (qPCR) analysis. (A) Targeting EHMT2 (B) Targeting SetDB1. (C) Bovine IFN-β (boIFN-β) levels in FBF cells stimulated with poly (I:C). mRNA levels were normalized to endogenous GAPDH and represented as fold increase compared to the control. The data represent mean ± SE from 3 independent experiments performed in duplicates. *P < 0.05, **P < 0.001 determined by 2-tailed Student's t-test.