FIG. 2.

Pharmacological inhibition of EHMT2 led to potent induction of boIFN-β in FBF cells. FBF cells were treated with the vehicle or EHMT2 inhibitor at 2 and 5 μM for 4 days. Four days after treatment, cells were stimulated with 0.5 μg/mL poly (I:C) and 6 h later, cells and supernatants were harvested for qPCR and chloramphenicol acetyltransferase (CAT)-ELISA (A) EHMT2 inhibitor dose–response on boIFN-β mRNA levels. (B) Quantitation of IFN levels in the supernatants by an Mx/CAT reporter gene assay. Units of boIFN-β per milliliter of the samples were calculated from a standard curve using recombinant human IFN-α2A (PBL Interferon Source). (C) Relative comparison in the antiviral activity in the supernatants as determined by IFN bioassay. The data represent mean ± SE from at least 2 independent experiments. *P < 0.05, **P < 0.001 determined by 2-tailed Student's t-test.