FIG. 3.

Pharmacological inhibition or depletion of EHMT2 in FBF cells resulted in an induction of IFN-stimulated genes (ISGs). FBF cells were treated with the EHMT2 inhibitor (5 μM) or vehicle for 4 days followed by 0.5 μg/mL poly (I:C) stimulation. The cells were harvested for RNA analysis by qPCR. Relative mRNA levels of (A) ISG-15, (B) 2′,5′-oligoadenylate synthetase 1 (OAS-1), (C) RIG-I, (D) myxovirus resistance 1 (Mx-1), (E) protein kinase R (PKR), and (F) MDA-5 in FBF cells stimulated with poly (I:C). For depletion of EHMT2, FBF cells were mock transfected or transfected with siRNAs targeting bovine EHMT2 or SetDB1. Forty-eight hours later, cells were stimulated with poly (I:C) (0.5 μg/mL) and 6 h later, cells were harvested for RNA analysis by qPCR. Relative mRNA levels of (G) ISG-15, (H) OAS-1, (I) Mx-1, and (J) PKR. The mRNA levels of these genes are expressed as fold increase over the transfection control. Independent gene expression was normalized with GAPDH as control. The data represent mean ± SE from 3 independent experiments. *P < 0.05, **P < 0.001, determined by 2-tailed Student's t-test.