FIG. 4.

Pharmacological inhibition or depletion of EHMT2 confers protection in bovine cells against foot-and-mouth disease virus (FMDV) and vesicular stomatitis virus (VSV) infections. FBF cells were treated with the EHMT2 inhibitor (5 μM) or vehicle for 4 days. Treated cells were unstimulated or stimulated with poly (I:C) (0.5 μg/mL) for 6 h, followed by infection with VSV or FMDV [multiplicity of infection (MOI) = 0.01]. Supernatants were collected at 36 or 41 hours postinfection (hpi) for determining viral titers (TCID₅₀). For depletion of EHMT2, FBF cells were mock transfected or transfected with siRNAs targeting bovine EHMT2 or SetDB1 for 48 h followed by infection with VSV. Thirty-six hours later, supernatants were collected for determining viral titers (TCID₅₀) in MDBK cells. (A) Left panel represents control FBF, exhibiting cytopathic effects (CPE). Right panel represents EHMT2 inhibitor-treated FBF monolayer. (B–D) Represents VSV and FMDV titers. Viral titers (log10) expressed as TCID₅₀ per milliliter. The data (mean ± SE) are from 3 independent experiments done in duplicates. **P < 0.001, ***P < 0.0001 determined by 1-way ANOVA followed by Tukey's multiple comparison test.