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. 2016 Jan 22;9:6. doi: 10.1186/s12920-016-0166-9

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© Tremblay et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Differential expression of innate immune inflammatory response genes in human necrotizing enterocolitis. Real-time qPCR analysis of transcript levels of selected target genes related to intestinal innate immunity. Ct values of selected genes were normalized using B2M as reference gene and data are expressed as r∆Ct values (reverse ∆Ct: Ct_{reference gene}-Ct_{gene of interest}) in order to display direct variation in NEC vs non-NEC controls (CTRL). Horizontal line represents the median value of r∆Ct values for CTRL and NEC samples. *: p <0.05 between CTRL and NEC samples. Numbers indicated represent the fold variation between NEC and CTRL