Fig. 2.

Validation of MIR494 cellular responses. a 769-P cells were seeded at 50,000 cells/well in a 24-well plate. Twenty-four hours post-seeding, cells were transfected with control mimic, MIR494 mimic, control antagomir, MIR494 antagomir in combination as indicated. Ninety-six hours post-transfection, protein lysates were collected, samples run on a SDS-PAGE gel, and analyzed via western blotting using the indicated antibodies. Three independent experiments were performed. b Representative light micrograph images are shown. c Representative light micrographs of 769-P cells treated with Ago2 siRNA and expressing MIR494 or control MIR are presented. d Protein lysates isolated from 769-P cells treated with Ago2 siRNA and expressing MIR494 or control MIR were analyzed by western blotting using the indicated antibodies. Three independent experiments were performed