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. 2016 Jan 22;16:6. doi: 10.1186/s12896-016-0238-0

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© Veith et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Cytogenetic characterization of PT1-CHO cell lines. a FISH analysis showing the different integration sites of the PT1 expression vector in the CHO-K1 genome. A centromeric integration site can be seen for PT1-30, and telomeric for PT1-55. FISH was performed on early passage (P10) recombinant PT1-CHO cell lines. Preparations were counterstained with DAPI (4′,6-Diamidine-2′-phenylindole dihydrochloride). Reverse DAPI conversions were performed to render latent G-banding images visible. Vector DNAs depicted were labelled by nick translation with red emission Dy-590-dUTP (PT1-7), or green emission Dy-495 (PT1-1/30/55) b Chromosome number (i.e. 2n = 22) ranged from 84 to 98 % as determined in 100 metaphases at passage P55.