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. 2016 Jan 22;16:6. doi: 10.1186/s12896-016-0238-0

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© Veith et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Transgene copy number and stability study in recombinant PT1-CHO cell lines. a Copy number of integrated transgenes was determined by quantitative PCR (qPCR) using primers on the light chain cDNA of PT1 construct at an early passage (P5). PT1-7 was used as a calibrator with a known copy number of one which was previously determined by Southern blot hybridization (data not shown). Samples were measured in triplicates. PT1-30 and PT1-55 revealed more than one copy of the transgene. Data represent means and standard error of the means (SEM) of n = 3 measurements. b A stability study was initiated with a relative value of 1 as a starting titer value. During the study, all clones showed instability to various degrees. In the most unstable clone PT1-1, a drop of productivity to 0.2 was measured whereas PT1-7 showed a loss of titer to only 0.8.