Figure 4.

Retrograde labeling in cholinergic cell groups Ch1–Ch8. Following injections of the retograde tracers in the dorsolateral striatum, retrogradely-labeled neurons were absent in Ch1, Ch2, Ch3, Ch7 and Ch8, and present in Ch4, Ch5 and Ch6, but co-expression with ChAT immunolabeling was only observed in Ch5 and Ch6. In these examples, neurons projecting to the striatum were labeled by the Ctb (red) and neurons projecting to the nucleus accumbens were labeled by the FG (green). The arrow indicates a neuron containing FG that is ChAT-negative. Images from Ch1 to Ch7 were obtained from coronal sections, whereas the image from Ch8 was obtained from a sagittal section. Scale bars: Ch1 to Ch8, 100 μm.