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. 2016 Jan 22;9:36. doi: 10.1186/s13104-016-1843-7

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© Pinheiro et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Schematic diagram of DNA constructs encoding canine IL-12 single-chain protein. The construct with canine native DNA is composed in tandem by nucleotide sequences encoding: signal peptide of p40, p40 mature protein, spacer, p35 mature protein and His-tag (a); the construct with DNA optimized for Trichoplusia ni translation is composed in tandem by nucleotide sequences encoding: GP64 signal peptide of AcMNPV, p40 mature protein, a spacer, p35 mature protein, spacer and His-tag (b)