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. 2016 Jan 22;9:36. doi: 10.1186/s13104-016-1843-7

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© Pinheiro et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Assessment of IFN-γ production by peripheral blood mononuclear cells (PBMC) stimulated with rsccaIL-12L. PBMC from six adult mongrel dogs were cultured for 48 h with: complete RPMI 1640 medium alone (medium), COS-7 cells supernatant containing canine IL-2 at 20 % (IL-2), purified rsccaIL-12L at 20 ng/mL (IL-12); or purified sccaIL-12L at 20 ng/mL combined with COS-7 cell SN-caIL-2 (IL-2 + IL-12). IFN-γ was assessed in the PBMC supernatants by capture ELISA. Data represent the average of duplicates and means obtained from six animals. Repeated measures ANOVA: p < 0.0001, Dunnett´s test *p < 0.05