Fig. 3.

Induction of TAA-specific T cell responses by DCs loaded with SNU899-derived Ags by various methods. a Induction of TAA-specific CD4⁺ T cells. TAA-loaded mature DCs were added to autologous PBLs at a ratio of 1:20. On day 6 after the second stimulation, the number of CD4⁺ T cells was assessed by flow cytometry. The methods used to load the DCs induced differential class II cross-presentation of TAAs to the T cells. However, the stressed lysis strategy resulted in the highest efficiency of T cell activation, reflecting a higher level of TAAs after loading. b Induction of TAA-specific CD8⁺ T cells. Similar to the cross-presentation by MHC class II, TCL-DCs were also much more efficient at cross-presentation of TAAs by MHC-I, and thus T cell activation, than DC-TCS or DC-ITC. Data are mean values ± SD of triplicate determinations. Statistical comparisons were performed using Student’s t-test. *P < 0.05 vs. DC control; **P < 0.01 vs. DC control