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. 2016 Jan 22;35:18. doi: 10.1186/s13046-016-0295-1

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© Wei et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Induction of TAA-specific CTL activity by three DC Ag-loading strategies. a Ag-loaded DCs were used as stimulators of autologous PBLs to compare their efficacy in CTL stimulation against Ag-pulsed target cells. PBLs and DCs were generated from a single donor. Data are from one representative experiment. b TAA-specific cytotoxicity was determined by flow cytometry. Data are the mean percentages of cytotoxicity ± SD of triplicate determinations. TCL-DCs induced a more potent CTL response compared with DC-ITC as shown by the increased cytotoxicity from SNU899-derived Ag-pulsed autologous DCs used as targets at a 10:1 E:T. Statistical comparisons were performed using Student’s t-test