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. 2016 Jan 22;35:16. doi: 10.1186/s13046-016-0291-5

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© Li et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Expression of NRP-1 in gastric cancer tissues and cells. a-c Representative images of section from gastric normal mucosa (a), adenocarcinoma (b) and undifferentiated cancer (c), immunostained with an anti-NRP-1 antibody. d Lysates of human gastric cancer cells were immunoblotted. e The expression of NRP-1 mRNA was analyzed by qRT-PCR, and normalized to β-actin. f The expression of NRP-1 and NRP-2 was examined in lysates of parental MGC-803, MGC-803-Sc and MGC-803-SUR^low cells by immunoblotting. NRP-1 expression in the three cell lines was further detected by immunohistochemistry