Fig. 6.
NRP-1 depletion influences the activation of VEGF/VEGFR2, EGF/EGFR and HGF/c-MET pathways. MGC-803 and MGC-803-NRPlow cells were transfected with siRNAs targeting VEGFR2 (a), EGFR (b) or c-MET (c). The cells were then cultured for 24 h in the presence or absence 100 ng/ml of recombinant VEGF-165 (a), of 10 ng/ml of recombinant EGF (b) or 100 ng/ml of recombinant HGF (c) proteins, respectively. Cell lysates were immunoblotted to determine the expression of key proteins involved in the above pathways as indicated. The density of each band was measured and normalized to β-actin. “*” indicates P < 0.05, and “**”, P < 0.001. “#” (P < 0.05) and “##” (P < 0.001) indicate a significant increase, and “†” (P < 0.05) and “††” (P < 0.001), a significant reduction, from untreated respective cells. “ϕ” (P < 0.05) and “ϕϕ” (P < 0.001) indicate a significant difference from MGC-803 cells with the same treatments