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. Author manuscript; available in PMC: 2016 Jan 22.
Published in final edited form as: J Immunol. 2015 May 6;194(12):5968–5979. doi: 10.4049/jimmunol.1402866

FIGURE 5.

FIGURE 5

Reporter gene analysis: the various α4 and β7 integrin reporter constructs of promoters from PM, RM, and SM were assayed after transfection as shown. Transfections were performed using AMAXA, and reporter gene expression was measured at 24–48 h for cell lines and within 6–24 h for the B-LCL. Experiments were repeated three times and data calculated. RA response element was used as a positive control and a GFP plasmid as an internal control in the assay to normalize for transfection efficiency. Data shown are expressed as mean ± relative luciferase units using the (A) CEMX174 cell line, (B) HUT78 cell line, (C) rhesus B-LCL, and (D) SM B-LCL. *p < 0.05.