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(a) Emission spectra and lifetimes (in ns in parentheses) for four common fluorophores: Alexa Fluor 680, 700, 750 and 790; data from [7]. (b) Spectral-temporal signatures associated with each fluorophore; each two-dimensional signature was concatenated into a vector. (c) Cramér-Rao lower bounds for root-mean-square (RMS) demixing error for three data types with all four fluorophores present. Lower values indicate better performance.
