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. Author manuscript; available in PMC: 2016 Jan 22.
Published in final edited form as: J Immunol. 2014 Nov 26;194(1):349–357. doi: 10.4049/jimmunol.1402330

Figure 1.

Figure 1

Generation of R314Q Nod2 knock-in mice. (A) Schematic of genomic, targeting, intermediate and final loci described in materials and methods. Δ, probe for Southern blotting; B, BglII site; * point mutation; F, FRT; Neo, neomycin resistance gene. (B) Southern blot analysis of genomic DNA digested with BglII and hybridized with the probe (Δ) shown in A. +/+, wild type; m/m, homozygous mutant; +/m, heterozygous mutant. (C) Chromatograms from sequence analysis of exon 4 from genomic DNA from +/+, +/m and m/m mice confirming the G to A base change (see squares in each panel) leading to a codon change from R to Q.