Fig 4. Triple immunofluorescent staining for SOX2, HIF-1α, and RNApII-S2P in zones around small pseudopalisading necroses or in areas showing no necrotic changes.

a: Glioblastoma tissue containing small pseudopalisading necrosis. Although SOX2⁺ and/or HIF-1α⁺ tumor cells were found, they were RNApII-S2P⁺. Therefore, SOX2⁺ HIF-1α⁺ RNApII-S2P^-/low cells were not observed. b: Glioblastoma tissue showing no necrotic changes. SOX2⁺ HIF-1α⁺ RNApII-S2P^-/low cells were not found. Insets at lower left of the panels a and b show higher magnification of the boxed areas in the merged images. Ns, small pseudopalisading necrosis; V, blood vessels; DIC, differential interference contrast image. Scale bars, 25 μm.