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. 2016 Jan 22;11(1):e0147480. doi: 10.1371/journal.pone.0147480

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© 2016 Gao et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — A: SDS-PAGE analysis of pGEX-4T-1–irisin expression and purification. M: low molecular weight marker (kDa); IN: induced culture; UI: un-induced culture; GST-Irisin: the purified eluent. B: Western Blotting of GST-irisin. IN: induced culture; UI: un-induced culture. C: Effect of GST-irisin on 3T3-L1 preadipocytes. 3T3-L1 preadipocytes were treated with various GST-irisin concentrations (0, 12.5, 25, 50, 100, and 200 nM), and the cell viability was assessed using the CCK-8 assay. D: qPCR of UCP1 of 3T3-L1-derived adipocytes. 3T3-L1 mature adipocytes were cultured with various GST-irisin concentrations (0, 50, 100, and 200 nM). The expression of UCP1 mRNA was determined by qPCR. Values are expressed as the mean ± SD of three independent experiments. *P < 0.05 versus control; **P < 0.01 versus control.