Fig 4. The effect of Cytl1 on the endometrial expression of LIF and HB-EGF.
(A) Reverse -transcription PCR analysis of LIF and HB-EGF mRNA expressions in human endometrial cell lines (HEC-1-A, RL95-2) after treatment with different concentrations of Cytl1. LIF expression in the culture supernatant of HEC-1-A (Ba) and RL95-2 (Bc), and HB-EGF expression in HEC-1-A (Bb) and RL95-2 (Bd) were detected by ELISA. The standard curve prepared according to manufacturer’s instruction was used to determine sample concentrations. P, progesterone. Data represent mean±SEM. *P < 0.05; **P < 0.01.