Fig 2. Intracellular signaling involved in osmotic and hypoxic induction of bFGF and HB-EGF gene expression.
A,B. Cellular levels of bFGF (A) and HB-EGF mRNAs (B) were determined with real-time RT-PCR analysis in cells cultured 6 h in iso- (control) and hyperosmotic (+ 100 mM NaCl) media, respectively. (C). The mRNA level was determined in cells cultured 24 h under control conditions and in the presence of CoCl2 (150 μM), respectively. The following pharmacological inhibitors were tested: the inhibitor of p38α/β MAPK activation, SB203580 (10 μM), the inhibitor of ERK1/2 activation, PD98059 (20 μM), the JNK inhibitor SP600125 (10 μM), and the inhibitor of PI3K-related kinases, LY294002 (5 μM). The vehicle control was made with dimethylsulfoxide (DMSO; 1:1000). Means ± SEM of 3–7 independent experiments using cells from different donors. Significant difference vs. unstimulated control: *P<0.05. Significant difference vs. NaCl control: •P<0.05.