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(A) Efficiency of NPC differentiation. Mouse Sox1-GFP ESCs were differentiated to NPCs for 6 days. Flow cytometry of the SOX1-GFP signal showing over 80% SOX1-GFP positive cells after differentiation. (B) ESCs and NPCs were subjected to polysome profiling and 12 fractions were collected into four groups. No translation F1-5), Low translation (F6-8), High translation (F9-11) and the bottom of the gradient (F12).
