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. 2016 Jan 22;11(1):e0147224. doi: 10.1371/journal.pone.0147224

Fig 1. Specificity of RT-qPCR amplification.

Fig 1

A) Melting curve of the 11 reference genes showing a single pick (each including three technical replicates of the cDNA pool of the total samples used in this study). B) Agarose gel (2%) showing amplification of a specific PCR product of the expected size for each gene tested in this study.