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Reactions were done using 25 mM pNP-Fuc as donor and 100 mM lactose as acceptor at 30°C and optimal pH. The concentrations were determined by HPAEC-PAD and for pNP by spectrophotometry. L-fucose (■), pNP (⧫), pNP-Fuc (X), 2’-FL (▲), and 3-FL (●).
