Table 2. Properties of metagenome-derived α-L-fucosidases and Thma.

	Mfuc1	Mfuc2	Mfuc3	Mfuc4	Mfuc5	Mfuc6	Mfuc7	Thma
pH opt	7	7	6	7	7	9	6	7 1
pH interval	6–9	6–10	6–8	6–9	5–8	6–10	6–7	4–9
Thermal half life
30°C	> 2 h	> 2 h	> 2 h	> 2 h	> 2 h	> 2 h	~98 min	> 2 h
40°C	> 2 h	> 2 h	> 2 h	> 2 h	> 2 h	~56 min	~28 min	> 2 h
50°C	> 2 h	~9 min	~67 min	> 2 h	< 2 min	~14 min	~8 min	> 2 h
60°C	< 2 min	< 2 min	~4 min	< 2 min	< 2 min	< 2 min	< 2 min	> 2 h
Kinetic parameters 2
Vmax	1.6±0.0	2.3 ±0.1	1.6 ±0.1	0.77±0.02	1.9±0.04	0.56±0.05	2.2±0.16	0.67±0.02
Km (Kprime)	0.11±0.01	0.14±0.01	0.31±0.03	0.071±0.01	0.13±0.01	0.40±0.05	0.28±0.05	0.06±0.01
Ki (h)	7.1±0.6	7.1 ±0.9	2.6 ±0.3	30 ±6.8	12±1.2	0.74±0.16	12 ±3.4	22 ±4.9

¹ pH optimum was measured with 0.1 and 1 mM substrate; for Thma two optima (pH 4 and 7) were observed with 1 mM substrate.

² Kinetic parameters were determined using pNP-Fuc as substrate at 30°C at the optimal pH.

Plots of pH vs. hydrolase activity, substrate concentration vs. hydrolase activity, and heating time vs. residual activity for the enzymes are presented in S1 Fig, S2 Fig and S3 Fig.