Table 3. Determination of regiospecificity and substrate specificity for hydrolysis of natural oligosaccharides and glycans.

	Mfuc1	Mfuc2	Mfuc3	Mfuc4	Mfuc5	Mfuc6	Mfuc7	Thma
2’-FL	76 ±1%	88±1%	2 ±1%	47±1%	87±1%	2 ±2%	94±1%	71±1%
3-FL	11 ±2%	4±2%	0 ±2%	3 ±2%	34 ±3%	0 ±2%	12 ±2%	1 ±2%
Citrus XG	4%	1%	1%	3%	39%	4%	3%	0%
Arabidopsis XG	100%	84%	7%	78%	100%	7%	43%	13%
Sambucus XG	100%	85%	7%	91%	100%	5%	44%	9%

The reactions were performed using 1 mM of substrate at 30°C for 150 min and the fraction of fucose released from the substrates under the reaction conditions employed is given in %. For hydrolysis of 2’-FL, 3-FL and citrus xyloglucan, the fucose content was determined by HPAEC-PAD. The hydrolysis of fucose from Arabidopsis thaliana and Sambucus nigra xyloglucan was measured using immuno-glycan arrays (S2 File), and 0% corresponds to the signal from untreated samples whereas 100% corresponds to the complete α-L-fucosidase-mediated removal of the antibody fucosyl epitope detected by the antibody.