Table 4. Fucosylated products from trans-fucosylation reactions.
| Enzyme | Concentration (mM) | ||||||
|---|---|---|---|---|---|---|---|
| pNP-Fuc | Fucose | 2’-FL | FL1 | Fuc-Fuc-pNP | |||
| α-1,2 | α-1,3 | α-1,4 | |||||
| Mfuc1 | 0.12 | 20.3 | 0.00 | 0.05 | 0.09 | 0.00 | 0.00 |
| Mfuc2 | 0.08 | 17.9 | 0.07 | 0.08 | 0.61 | 0.00 | 0.00 |
| Mfuc3 | 0.00 | 18.3 | 0.00 | 0.02 | 0.13 | 0.03 | 0.00 |
| Mfuc4 | 0.44 | 18.3 | 0.00 | 0.04 | 0.18 | 0.00 | 0.00 |
| Mfuc5 | 0.45 | 12.9 | 0.03 | 0.24 | 0.49 | 0.20 | 0.04 |
| Mfuc6 | 1.17 | 7.3 | 0.00 | 0.01 | 5.67 | 0.32 | 0.75 |
| Mfuc7 | 4.58 | 12.3 | 0.00 | 0.02 | 1.17 | 1.99 | 0.00 |
| Thma | 1.02 | 13.9 | 0.19 | 0.00 | 3.40 | 0.06 | 0.06 |
1 FL is fucosyllactose other than 2’-FL and 3-FL.
The reactions were performed at 30°C for 50 min using 20 mM pNP-Fuc as donor and 25 mM lactose as acceptor. Concentrations of substrates and products in trans-fucosylation reactions was determined using HPAEC-PAD and NMR. Concentrations of pNP-Fuc and and Fuc- Fuc-pNP was estimated by comparison of characteristic non-over-overlapping 1H NMR signals and that of L-fucose.