Fig 12. Expression of a dominant-negative form of Hsp70 influences the persistence of Cyc8^4KtoR inclusions.

(A) Cells expressing Cyc8^4KtoR-GFP from the endogenous CYC8 locus and carrying either an empty 2 micron vector or a 2 micron vector with an dominant-negative form of Hsp70 (SSA1) were exposed to a time course of hyperosmotic stress (1.2M sorbitol), fixed at the times indicated, and imaged by fluorescence microscopy. (B) Analysis of data from (A). Six fields of cells for each condition, with at least 40 cells/field, were counted for the presence of cytoplasmic inclusions. Data represent the average percentage of cells that contained cytoplasmic inclusions within the six fields. Error bars are the standard deviation. A two-tailed, heteroscedastic student’s t-test was used to determine significance.