Fig. 5.
Expression of interested cytokines in the gingivae of ligated female and male mice. Histochemistry was used to confirm gingival IL-17 expression (A,B). (A) Representative fluorescent confocal images of IL-17 were shown (Green); and (B) the fluorescence intensities of these and additional representative images from independent mice (3 per group) were quantified using Neurolucida. Asterisks indicate statistically significant (p < 0.05) differences between ligated female and male mice. Representative fluorescent confocal images of IL-1β (C) (Blue) and IL-6 (E) (Red) were also shown; and the fluorescence intensities of IL-1β (D) and IL-6 (F) from independent mice (3 per group) were quantified.