Fig 2. Small RNA sequencing and RT-qPCR reveal high level of expression of miR-134 in HG-dfECFCs and dmECFCs.

(A) Differentially expressed miRNAs between HG-dfECFCs and dfECFCs. Numbers of dominant miRNAs in each ECFC type (fold change > 2) are shown. Y-axis was shown the fold ratio of RPM of HG-dfECFCs against dfECFCs. (B) The top ten HG-enriched miRNAs are shown. Y-axis was indicated the log₂ ratio of HG-dfECFC against dfECFCs. The diameters of the circles indicate the log₂RPM of the HG-dfECFCs. (C) The putative HG-induced miRNAs were validated using dfECFCs treated with NG and HG by RT-qPCR. * p < 0.05, ** p < 0.01, *** p < 0.001 by one-way ANOVA followed by Tukey’s post-hoc test. (D) The HG-induced miRNAs were also analyzed in dmECFCs and dfECFCs by RT-qPCR. * p < 0.05, ** p < 0.01 by one-way ANOVA followed by Tukey’s post-hoc test.