Fig. 3.

OPG decreases tumor development and increases animal survival in EWS tumor models in mice. Tumors were induced on Nude mice by transplantation of a fragment of TC-71 tumor in close contact with the tibia as described in Section 2. Groups of 6 mice were assigned as pcDNA3.1 (empty pcDNA3.1 plasmid/F68 reagent) and hOPG (pcDNA3.1.3-hOPG1-194/F68 reagent). The F68/DNA formulations were administered through intrasmuscular injection into the 2 tibial anterior muscles weekly, beginning one week before tumor transplantation. (A) Mean tumor volume per group. (B) Overall survival for the 2 groups over a 42 days period. (C) Tumor volume evolution of individual mice.