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Origin of RANKL present in the tumor microenvironment. Tumor and muscle lysats, and serums of mice bearing Ewing's sarcoma were analyzed for the production of human and mouse RANKL using species-specific ELISA detection Kits. Results are expressed as ng/mL for serum detection and ng/mg of protein for detection on muscle or tumor lysat.
| Serum (ng/mL) | Tumor (ng/mg) | Muscle (ng/mg) | |
|---|---|---|---|
| mRANKL | 0.05 | 0.2 | 0.1 |
| hRANKL | 1.2⁎⁎⁎ | 4.4⁎⁎⁎ | 1.4⁎⁎⁎ |
p<0.001.
