Fig. 2.

Dexamethasone enhances syndecan-1 (SDC1) expression in MCF-7 cells. For analyzing DEX-effects MCF-7 cells were serum starved 12 h prior to and during treatment. MCF-7 cells were treated with (A) different doses of dexamethasone (DEX) for 72 h or with (B) 10⁻⁶ M DEX for 24, 48, and 72 h. Afterwards the SDC1 expression was analyzed using real-time PCR. (C) The receptor antagonist RU-486 (10⁻⁵ M) was used to inhibit the DEX effects in MCF-7 cells. To analyze the protein expression of SDC1 in more detail we used (D) immunofluorescence staining for the membrane bound form and (E) ELISA for the soluble form. SDC1 is displayed in green, the nucleus in blue. N=3, ^⁎p<0.05, ^⁎⁎⁎p<0.001, magnification 400×, DEX—dexamethasone, RU—RU-486, and SDC1—syndecan-1.