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OPG is up-regulated after SDC1 knock-down in MCF-7 cells. After SDC1 knock-down cells were cultured in medium without FCS for 36 h. OPG expression was analyzed using (A) real-time PCR and (B) ELISA. N=4, ⁎p<0.05, ⁎⁎⁎p<0.001, SDC1 kd—syndecan-1 knock-down, and scr. C—scrambled control.
