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Time course of growth of cells transfected with pMD-KIP1 expressing p27KIP1. HCC-9204 cells were induced by 100 μmol/L ZnSO4. HCC-9204 cells viability was determined by microscopic examination of cells stained by 1 g/L trypan blue, counting cells on a hemocytometer at the time point indicated. Each treatment was triplicate.
