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. 2000 Aug 15;6(4):546–552. doi: 10.3748/wjg.v6.i4.546

Figure 1.

Figure 1

Expression of CD45 and ANK61 antigen on hepatic NK cells (pit cells). The cells were washed out of the liver by sinusoidal lavage. After Ficoll-Paque gradient centrifugation and nylon-wool adherence to remove erythrocytes, granul ocytes, monocytes and B lymphocytes, the cells were stained with anti-NKR-P1A mAb (3.2.3) (phycoerythrin) and anti-ANK61 antigen (ANK61), anti-CD45 mAb (ANK 74) (fluorescein) and analyzed by two-color flow cytometry. CD45 and ANK61 anti gen were expressed on the x-axis (fluorescein), NKR-P1A on the y-axis (phycoe rythrin).