Table 1.
Details of antisera and main reagents used
| Antisera against | Working dilution | Code | Specificity | Source |
| Synthetic human gastrin | 1:5000 | GP-1304 | No cross reaction with cholecystokinin-8 | Dr. N Yanaihara,Shizuoka |
| Leucine-enkephalin | 1:80000 | 1671 | — | UCB-Bioproducts Bruxelles |
| Bovine pancreatic polypeptide | 1:12000 | 615-R-110 | Cross-react s with human pancreatic polypeptide | Dr. RE Chance, Indianapolis |
| Substance P | 1:2000 | MAS 035B | — | Sera-Lab., Sussex |
| Gastric inhibitory polypeptide | 1:10000 | G/R/34-IIID | No cros s reaction with glucagon | Dr. D Grube, Hannove |
| Porcine glucagon | 1:1000 | RPN1602 | Wholly cross react with pancreatic | Amersham International pl., |
| and intestinal glucagon | Amersham | |||
| Synthetic human cyclic | ||||
| somatostatin | 1:3000 | — | — | Dr. S Ito Niigata |
| Glucagon-like immunoreactants | 1:1000 | RPN1604 | Wholly cross react with pancreatic | Amersham International pl., |
| and intestinal glucagon | Amersham | |||
| Insulin | 1:1000 | 47291 | — | — |
| Avian pancreatic polypeptide | 1:10000 | Iance-10/5/81 | No cross reaction with glucagon | Dr. JR Kimmel, Kansas City |
| 5-Hydroxytriptamine | 1:10000 | Lot.16302 | — | Immunonuclear Corp.Stillwater |
| Synthetic porcine motilin | 1:1000 | R-1104 | Reacts against entire molecules | Dr. N Yanaihara, Shizuoka |
| Natural porcine | ||||
| cholecystokinin-33 | 1:3000 | — | Reacts with cholecystokinin 11-20, | Dr. J Yamada, Shizuoka |
| no cross reaction with gastrin | ||||
| Synthetic porcine secretin | 1:1000 | R-801 | Reacts with the C- and N-terminals | Dr. N Yanaihara, Shizuoka |
| Synthetic bovine neurotensin | 1:1000 | R-3501 | Dr. N Yanaigar a, Shizuoka | |
| Synthetic porcine vasoac tive | ||||
| intestinal polypeptide | 1:2000 | R-502 | Reacts against entire molecules | Dr. N Yanaihara, Shizuoka |
| Bombesin | 1:3000 | 27070 | Cross reaction with GRPImmunonrclear | Corp. Stillwater |
| Neuron specific enolase | 1:1000 | — | — | Dako, Copenharge |
| Prochymosin | 1:2000 | — | — | A. Andren, Uppsala |
| Pepsinogen | 1:2000 | — | — | A. Andren, Uppsala |
| Rabbit PAP | 1:100 | Z - 113 | — | Dako, Copenharge |
| Guineapig PAP | 1:50 | 24699 | — | Dako, Copenharge |
| PAP Kit | 1:100 | 61- 2003 | — | Zymed Lab. Inc., SouthSan |
| Francisco, USA | ||||
| ABC Kit | 1:50 | PK-4001 | — | Vector Lab. Burlingame USA |