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. 2015 Aug 4;31(1):69–81. doi: 10.1093/mutage/gev055

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Published by Oxford University Press on behalf of the UK Environmental Mutagen Society 2015.

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Figure 5. — Generation of γH2AX foci in DT40 cells and micronucleus formation in CHO-K1 cells treated with 2-oxiranemethanamine. (A) Dose-dependent γH2AX foci (red) formation in DT40 cells. The indicated DT40 clones were treated with 2-oxiranemethanamine for 24h. Images were acquired in ImageXpress using a 40× objective. Hoechst staining (blue) indicates DNA. (B) Micronucleus formation in CHO-K1 cells. CHO-K1 cells were treated with 2-oxiranemethanamine for 24h without S9 treatment. Hoechst staining (blue) indicates DNA. Images were acquired in ImageXpress using a 20× objective. Arrows indicate a cell with MN.