Figure 1.

Induction and Development of Pure, Stage-Specific Gametocytes

(A) Gametocyte production protocol. To create stages I–IV, all steps were performed, but to create stage V only, steps indicated by red boxes were omitted.

(B) Simplified schematic flow chart for the SaLSSA gametocyte assay.

(C) Giemsa smears of gametocytes of stage I-V and gametocyte counts and percentages for each stage based on their morphology (numbers pooled from multiple cycles).

(D) Z prime values for the different gametocyte stages and assay protocols. ¹384-well TSSA, stages I-V, ²384-well-SaLSSA, stages I-V, ³1,536-well SaLSSA, stage V.

(E) Displayed is the number of viable stage V gametocytes in a dilution series over time (0.5 hr until 6 hr). These data were acquired using stage V gametocytes and 1,536-well SaLSSA (R² > 0.99).