Induction and Development of Pure, Stage-Specific Gametocytes
(A) Gametocyte production protocol. To create stages I–IV, all steps were performed, but to create stage V only, steps indicated by red boxes were omitted.
(B) Simplified schematic flow chart for the SaLSSA gametocyte assay.
(C) Giemsa smears of gametocytes of stage I-V and gametocyte counts and percentages for each stage based on their morphology (numbers pooled from multiple cycles).
(D) Z prime values for the different gametocyte stages and assay protocols. 1384-well TSSA, stages I-V, 2384-well-SaLSSA, stages I-V, 31,536-well SaLSSA, stage V.
(E) Displayed is the number of viable stage V gametocytes in a dilution series over time (0.5 hr until 6 hr). These data were acquired using stage V gametocytes and 1,536-well SaLSSA (R2 > 0.99).