Figure 3.

Diosgenin inhibits the expression of α-SMA and collagen type I in TGF-β1-induced HSC-T6 cells. HSC-T6 cells were stimulated with TGF-β1 after treatment with various concentrations of diosgenin for 24 h. RT-PCR was performed to detect the mRNA expression levels of collagen type I (A) α-SMA (B); (C) Western blot analysis was performed to detect the protein expression levels of α-SMA and collagen type I using the antibodies indicated. Values were expressed as mean ± SD of three independent experiments. *P<0.05 compared with the control group. #P<0.05 compared with the TGF-β1 group.