Figure 4.

Diosgenin inhibits the expression of TGF-β receptor I and II in TGF-β1-induced HSC-T6 cells. (A) HSC-T6 cells were stimulated with TGF-β1 after treatment with various concentrations of diosgenin for 24 h. Western blot analysis was performed to detect the protein expression levels of TGF-β RI and II using the antibodies indicated. The relative protein expression levels of TGF-β RI (B) and II (C) were quantified using Image-Pro Plus 6.0 software and normalized to β-actin. Values were expressed as mean ± SD of three independent experiments. *P<0.05 compared with the control group. #P<0.05 compared with the TGF-β1 group.