Table 1.

Primer sequences used for qRT-PCR

Gene	Size (bp)	Sense primer	Anti-sense primer
NALP3	169	5’-AACAGCCACCTCACTTCCAG-3’	5’-CCAACCACAATCTCCGAATG-3’
ASC	75	5’-CTCCTCAGTCGGCAGCCAAG-3’	5’-TTGTGACCCTCGCGATAAGC-3’
Caspase-1	146	5’-GCACAAGACCTCTGACAGCA-3’	5’-TTGGGCAGTTCTTGGTATTC-3’
IL-1β	107	5’-AACCTCTTCGAGGCACAAGG-3’	5’-GGCGAGCTCAGGTACTTCTG-3’
IL-18	142	5’-GATTACTTTGGCAAGCTTGAA-3’	5’-ATATGGTCCGGGGTGCATTA-3’
GAPDH	112	5’-GAAGGTGAAGGTCGGAGTC-3’	5’-GTCAATGAAGGG GTCATT-3’

Note: NALP3, Natch domain, Leucine-rich repeat and PYD-containing protein 3; ASC, apoptosis-associated speck-like protein containing a CARD; Caspase-1, Cysteiny aspartate-specific protease-1; IL, interleukin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.