Table 1.
Primers used to amplify regions in CYP2C9
| Primer name | Primer sequence (5’-3’) | DNA size for PCR (bp) |
|---|---|---|
| PromoterF | GCAGTGATGGAGAAGGGAGA | 924 |
| PromoterR | AATTCGGTGTGTGCCTCTTT | |
| Exon1_F | GAACCATCTGGGTTAACATTTG | 925 |
| Exon1_R | AGTGCATTCTTGGCACCAT | |
| Exon2_3_F | TGCCTTGAACATCACAGGCCATC | 826 |
| Exon2_3_R | TGGCTCTCAGCTTCAAACCCCC | |
| Exon4_F | TCTTGCCCTTTCCATCTCAG | 911 |
| Exon4_R | TGCCACATAATACGACAAAGTG | |
| Exon5_F | TGCTGTCATCTACAAAACGTGA | 908 |
| Exon5_R | CAGGGATTTGACTTCTTCCTTG | |
| Exon6_F | AATCCCAGGATGGGGTCTAC | 880 |
| Exon6_R | CAATTGATTCCAGTGCCTCA | |
| Exon7_F | TTTGATTGGAGATTTTATTCCATTT | 925 |
| Exon7_R | GATTCAGTTCTTTCCAAACTAGCC | |
| Exon8_F | TACTGCCCTTCTTTGGAACGGGAT | 809 |
| Exon8_R | ACCTCCCAACCCCCAACAGC | |
| Exon9_F | AGAATGTGAGGGTCCAGATCA | 913 |
| Exon9_R | TTCAGGGAAGGGAAAATGTG | |
| 3’-UTR_F | TGTGGGAGAAGCCCTGGCCG | 737 |
| 3’-UTR_R | AGAGCTGCCCCTGGACACAG |
PCR: polymerase chain reaction.