Table 1.
Sequences and locations of primers used in this study
| Primer name & amplication | Sequence (5′ to 3′) | Construct length (bp) | |
|---|---|---|---|
| ProI promoter assay | |||
| Forward primer | |||
| 2DL1-For | AAACTCCAGAATTTACAGGTGGGG | ||
| 2DS1-For | AAAATCCAGAGTTTAAATGTGTGG | ||
| 3DL1-For | AAAATCCAGAATTTACATGTTGTG | ||
| 2DL5-For | AAACTCCAGAATTTACAGGTGTGG | ||
| 2DL2/3DL2-For | CTTGAGACAGACTCTTCCTCTGTCAC | ||
| 2DL4-For | CAGACCTTCAATTGACATATTGTG | ||
| 3DL3-For | CTGGGAGGTGGAGGCTGCAATGAG | ||
| Reverse primer | |||
| 2DL1/3DL1-Rev | GAGATTCAAACTCTTCTTCCTGTG | ||
| 2DL2/2DS2-Rev | GAGATTCAAACTCTTCTTCATATG | ||
| 2DL4-Rev | GAGATTCAAACTCTCCTTGATATG | ||
| 3DL2-Rev | GAGATTCAAACTTTTCTTCATGTG | ||
| 3DL3-Rev | GAGATTCAAACTCTTCTTCATGTG | ||
| 2DL2 5'-deletion assay | |||
| 2DL2-1527-For | ACAGGTGTTTATTGAACCAAC | 1,001 | |
| 2DL2-1320-For | GAGACAGTCTCACTCTCTCACC | 794 | |
| 2DL2-1240-For | AGCGATTGTCTTCCCTCAGCCTCC | 714 | |
| 2DL2-1141-For | GAGACGGGGTTTCAGAATGTTGG | 615 | |
| 2DL2-953-For | CACCCAGGTATAGTGAAGGACC | 427 | |
| 2DL2-815-For | CCTACGTGTAACCCTATGGTCC | 289 | |
| 2DL2-764-For | CCACCTTAAACTACATGGCAGCC | 234 | |
| 2DL2-527-Rev | GGTGACAGAGGAAGAGTCTGTCTC | ||
| 2DL2 3'-deletion assay | |||
| 2DL2-953-For | CACCCAGGTATAGTGAAGGACC | ||
| 2DL2-589-Rev | CAAATCAGAGGACTCAGGTTACC | 365 | |
| 2DL2-566-Rev | GCAAACTAAATAACCTATAATAACAAATCAGAGG | 388 | |
| 2DL2-527-Rev | GGTGACAGAGGAAGAGTCTGTCTC | 427 | |